The ability to produce chiral amines using an enzyme and ammonia as the amine source is still an arduous journey. A few of the major hurdles to this pursuit are:
1) The limited substrate scope of most of the enzymes.
2) The poor stability of dehydrogenases in an organic solvent.
EnzBond has utilized its technology to develop a class of dehydrogenases that are able to:
EnzBond was able to discover these enzymes in just one round of evolution with less than 4 months of development time.
Furthermore, EnzBond discovered an enzyme with a sequence homology of 40% to target parental sequence that displayed high activity and stability to the Bi-Phenyl substrate in 25% DMSO.